APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:10000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full length of human Glutamine synthetase.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutamate-ammonia ligase , GLNS , GS , PIG43 , PIG59
Cellular Localization
Cytoplasm, cytosol,Microsome,Mitochondrion,Cell membrane
Background
The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Database
Research Area
DATA IMAGES
|
GTX638336 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX638336 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX638336 IHC-P Image
Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX638336 IHC-P Image
Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX638336 IHC-P Image
Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat cerebellum.Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX638336 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX638336 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
GTX638336 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
|
|
GTX638336 ICC/IF Image
Glutamine synthetase antibody [HL2283] detects Glutamine synthetase protein at glia by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glutamine synthetase stained by Glutamine synthetase antibody [HL2283] (GTX638336) diluted at 1:250.Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
|
REFERENCE
There are currently no references for Glutamine synthetase antibody [HL2283] (GTX638336). Be the first to share your publications with this product.
REVIEW
Glutamine synthetase antibody [HL2283]
Cat. No. GTX638336
Rating
|
|
( Average 5 based on 1 users reviews)
|
Application
IHC (Paraffin sections)(IHC-P)
|
|
( Average 5 based on 1 users reviews)
|
Date :
Anonymous submitted on 22-Aug-2024
Application Tested :
IHC-P
Sample Species :
Rat
Sample :
Rat iver tissue
Fixation :
4% PFA
Permeabilization /Antigen retrieval :
Citrate Buffer
Primary Antibody :
1:500, 4°C, >12Hr